Incorporating an exciting new technology, phusion dna polymerase brings together a novel pyrococcuslike enzyme with a processivityenhancing domain. Thermo scientific phusion highfidelity dna polymerase offers extreme performance for all major pcr applications. Please let us know if there is anything we can do to help your research. Phusion highfidelity pcr kit f553s 50 reactions in 50 l volume f553l 200 reactions in 50 l volume thermo scienti. Thermo scientific phusion highfidelity pcr master mix. All trademarks mentioned in the operating instructions are the. Bioke has implemented multiple measures to keep our employees and business partners safe during the covid19 outbreak, and at the same time to maintain the continuity of supply of our products and support to our customers. Thermo scientific phusion green gc buffer pack fisher. Thermo scientific phusion highfidelity pcr kit 500u. Perform experiments the way you want with the thermo scientific evolution 201 and 220 spectrophotometers. The product manual includes details for how to use the product, as well as details of its formulation and quality controls. Phusion dna polymerase 2 ul 5x phusion hf buffer 5x phusion gc buffer dmso 50 mm mgcl 2 solution store at 5 to 30c.
Thermo scientific easypure ii rf 21 section 2 introduction the thermo scientific easypure ii rf is a batchfed water purification system designed to provide type i reagentgrade water with extremely low organic carbon content. Phusion highfidelity pcr master mix with gc buffer neb. Browse by field and technique below, or use the search function above. Phusion is a registered trademark and property of thermo fisher scientific. The phusion highfidelity dna polymerase should be pipetted carefully and gently as the high glycerol content 50% in the storage buffer may otherwise lead to pipetting errors. New england biolabs uk ltd phusion highfidelity pcr kit. Easypure ii reservoir feed water purification system. Thermo scientific phusion green hf buffer pack buffer packs for use in pcr with phusion highfidelity dna polymerases. Phusion highfidelity dna polymerase is supplied with 5x phusion hf buffer, 5x phusion gc buffer, dmso and 50 mm mgcl2 solution. Kit phusion highfidelity pcr kit product introduction ideal for high speed and highperformance pcr. Thermo scientific phusion sitedirected mutagenesis kit, point mutations, insertions and deletions can be introduced in any type of plasmid dna. Both phusion hf buffer and phusion gc buffer provide 1. Phusion hot start ii high fidelity dna polymerase is the most accurate hot start dna polymerase on the market. Thermo scientific gallery brings excellent analytical performance to colorimetric, enzymatic and electrochemical measurements in a compact and affordable design.
Hf buffer for phusion dna polymerases two optimized buffers are available for thermo scientific phusion highfidelity dna polymerases. Thermo cl2 manual mazda 323 c repair manual productspecifications thermo scientific cl2 1999 jeep wrangler sahara manual thermo iec centrifuges and centra cl3r and pdf manual thermo scientific cl2 manuals suzuki z250 quad sport manual thermo scientific manuals biosurplus. Gallery covers a wide range of application areas, e. Product information thermo scientific phusion site. Installation and operation manual thermo fisher scientific. Phusion highfidelity pcr master mix with gc buffer offers robust performance and can be used for all pcr applications.
This calculator generates a pipetting table for setting up pcr reactions with thermo scientific phusion dna polymerases. The annealing gradient should extend up to the extension temperature twostep pcr. Thermo 2837 digital water bath marshall scientific. Thermo scientific phusion green highfidelity dna polymerase 2 ul the most accurate thermostable dna polymerase available offering superior performance for cloning and other applications requiring high fidelity. Thermo scientific revco blood bank refrigerators installation and operation 3 unpacking and inspection at delivery, examine the exterior for physical damage while the carriers representative is present. This kit uses the highly processive thermo scientific phusion hot start ii highfidelity dna polymerase for exponential pcr amplification of dsdna plasmid to be mutated. Thermo scientific revco blood bank refrigerators installation and operation 3 unpacking and inspection at delivery, examine the exterior for physical damage while the. Thermo scientific revco laboratory refrigerators and freezers.
Phusion dna polymerase in phusion hf buffer is determined to be 4. Due to the unique nature of phusion dna polymerases, please pay special attention to the guidelines listed below. More details can be found in the technical manuals for phusion dna polymerases. Phusion highfidelity pcr master mix with hf buffer is a 2x master mix consisting of phusion dna polymerase, deoxynucleotides and reaction buffer that has been optimized and includes mgcl 2. Phusion dna polymerase was developed by finnzymes oy, now a part of thermo fisher scientific. Phusion dna polymerase possesses the following activities. Phusion highfidelity dna polymerase new england biolabs. Thermo scientific epijet bisulfite conversion kit is designed for simple and reliable bisulfite conversion of dna for methylation analysis.
Phusion highfidelity dna polymerase high performance for. Robust reactions maximal success with minimal optimization. Its unique structure, a novel pyrococcuslike enzyme fused with a processivityenhancing domain, increases. Page 1 installation and operation manual thermo scientific thermolyne furnace benchtop industrial type fd1500m, type models. Thermo scientific phusion green highfidelity dna polymerase. It uses a threestage deionization process combined with a 0. Installation and operation manual thermo scientific revco. Shop online for a wide selection of fisher scientific isotemp programmable ovens offer exceptional performance, safe operation and precision control. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged. The thermo scientific phusion highfidelity dna polymerase offers high performance for all major pcr applications.
Annealing rules for phusion dna polymerases are different from many common dna polymerases such as taq dna polymerases. Two optimized buffers are available for thermo scientific phusion highfidelity dna polymerases. Manufactured and qualitycontrolled at new england biolabs, thermo scientific phusion highfidelity dna polymerase offers both high fidelity and robust performance. Datasheet for phusion highfidelity dna polymerase m0530. Affibody inhibits the dna polymerase activity at room temperature and thus prevents the amplification of nonspecific products. Thermo scientific revco laboratory refrigerators and freezers unrivalled sample protection researchers worldwide protect more than two billion samples inside thermo scientific cold storage equipment. Thermo scientific phusion green hf buffer pack phusion. It combines the phusion dna polymerase and a reversibly bound, specific affibody ligand. Evolution 300600 uvvis spectrophotometer user guide. Due to the nature of the phusion highfidelity dna polymerase, the optimal reaction conditions may differ from pcr protocols for standard dna polymerases.
Thermo 2837 digital water bath the thermo 2837 digital water bath has a 12l capacity with a temperature range up to 99. The phusion dna polymerase generates long templates with an accuracy and. Thermo scientific phusion buffers choose between two buffer packs to optimize highfidelity pcr or difficult or long template amplification using thermo scientific phusion highfidelity dna polymerases. Thermo scientific user manuals download manualslib. Thermo scientific heratherm 11 1 safety notes basic operating precautions these operating instructions describe heratherm ovens. Pcr reaction setup for phusion thermo fisher scientific us. Thermo scientific phusion hot start ii dna polymerase 2 u.
Since their introduction, thermo scientific phusion highfidelity dna polymerases have been referenced in thousands of publications for highperformance pcr and have become the choice for a multitude of demanding applications ranging from reconstruction, design and massivelyparallel, highthroughput sequencing of whole genomes. Highperformancegravityconvection doubledoor chambervolume 2. Phusion dna polymerases offer robust performance with short protocol times, even in the presence of pcr inhibitors, and generate higher yields with lower enzyme amounts than other dna polymerase. Thermo scientific phusion highfidelity pcr kit life. Phusion highfidelity pcr master mix with hf buffer neb. It is a 2x master mix consisting of phusion dna polymerase, deoxynucleotides and reaction buffer that has been optimized and includes mgcl 2. All that is required is the addition of template, primers and water. Heratherm ovens have been manufactured to the latest state of the art and been tested. If necessary, use a temperature gradient to find the optimal annealing temperature for each templateprimer pair combination. Powerful software, cuttingedge technology and an array of accessories consistently delivers the high quality results you expect. Fill in the volume of template dna you want to use, the number of pcr reactions you intend to run, and the individual reaction volume. By using the same innovative software for onboard and computer control, your instrument is always up to.